欧亚国际

最近，科学家利用VideometerLab多光谱成像系统发表了题为Single and Double Mutations in Tomato Ripening Transcription Factors Have Distinct Effects on Fruit Development and Quality Traits的文章，文章发表在知名期刊Front Plant Sci.上。 

Fruit Trait Phenotyping

Fruit trait data were collected across four field seasons (2016,2017, 2018, and 2020). The genotypes, developmental stages,number of biological replicates, and number of field seasons used for fruit trait phenotyping can be found inSupplementary.Table 2. One season of phenotyping was performed for Cnr/rinand rin/nor double mutant fruits for color, firmness, and ethylene. Three seasons of data were collected for the Cnr/nor double mutant fruit for ethylene and two seasons of data for color and firmness. Fruit were collected from multiple plots or harvests to capture  environmental variability. Fruit trait measurements were taken on the same day of harvest for all samples unless noted. Intact and halved fruit were imaged using the VideometerLab 3 (Videometer, Denmark).

Single and Double Mutations in Tomato Ripening Transcription Factors Have Distinct Effects on Fruit Development and Quality Traits

Jaclyn A Adaskaveg 1, Christian J Silva 1, Peng Huang 1, Barbara Blanco-Ulate 1

Affiliations expand

PMID: 33986762

PMCID: PMC8110730

DOI: 10.3389/fpls.2021.647035

Front Plant Sci. 2021 Apr 27;12:647035.doi: 10.3389/fpls.2021.647035. eCollection 2021. 

Abstract

Spontaneous mutations associated with the tomato transcription factors COLORLESS NON-RIPENING (SPL-CNR), NON-RIPENING (NAC-NOR), and RIPENING-INHIBITOR (MADS-RIN) result in fruit that do not undergo the normal hallmarks of ripening but are phenotypically distinguishable. Here, we expanded knowledge of the physiological, molecular, and genetic impacts of the ripening mutations on fruit development beyond ripening. We demonstrated through phenotypic and transcriptome analyses that Cnr fruit exhibit a broad range of developmental defects before the onset of fruit ripening, but fruit still undergo some ripening changes similar to wild type. Thus, Cnr should be considered as a fruit developmental mutant and not just a ripening mutant. Additionally, we showed that some ripening processes occur during senescence in the nor and rin mutant fruit, indicating that while some ripening processes are inhibited in these mutants, others are merely delayed. Through gene expression analysis and direct measurement of hormones, we found that Cnr, nor, and rin have alterations in the metabolism and signaling of plant hormones. Cnr mutants produce more than basal levels of ethylene, while nor and rin accumulate high concentrations of abscisic acid. To determine genetic interactions between the mutations, we created for the first time homozygous double mutants. Phenotypic analyses of the double ripening mutants revealed that Cnr has a strong influence on fruit traits and that combining nor and rin leads to an intermediate ripening mutant phenotype. However, we found that the genetic interactions between the mutations are more complex than anticipated, as the Cnr/nor double mutant fruit has a Cnr phenotype but displayed inhibition of ripening-related gene expression just like nor fruit. Our reevalsuation of the Cnr, nor, and rin mutants provides new insights into the utilization of the mutants for studying fruit development and their implications in breeding for tomato fruit quality.

Keywords: CNR; NOR; RIN; abscisic acid; ethylene; fruit quality; fruit ripening; fruit senescence.

Copyright © 2021 Adaskaveg, Silva, Huang and Blanco-Ulate.