欧亚国际

SMO是欧洲先进的机械设备制造与设计工程公司，在机械自动化以及机器视觉成像领域拥有丰富的设计和实践经验，为欧洲先进客户提供机械解决方案，SMO公司将机械领域的先进理念带入了植物表型研究领域，所采用的配件均为工业界广泛认可的高品质配件，耐受苛刻环境，另外表型设备领域的好多自动化配件，均由SMO公司自主设计，例如WIWAM系统的高精度称重浇水工作站，专有的高精度相机定位系统等等，鉴于工业领域的丰富经验，可针对不同客户需求，提供真正快速定制化的解决方案。因公司拥有较为强大的工程师团队，一般数周左右就可以提供较复杂表型成像系统的解决方案。由于采用开放式框架结构，目前WIWAM可以集成目前市面上所有的相机传感器模块，如RGB相机、叶绿素荧光成像模块、高光谱相机模块、近红外相机模块、3D激光扫描模块、多光谱模块、CT成像模块等，是目前上表型成像领域整合能力先进的公司，这也顺应了植物表型组织提出的标准化的潮流，提供设备涉及到室内表型、田间表型、根系表型、种子表型等领域。在该领域较突出的一点，SMO公司是目前所有表型设备提供商里不多见的进行自主机械、控制系统设计和生产的公司，因自有长期的机械工程人员和自己的生产场地，能应对表型领域客户的较为多样化的需求。

摘要

干旱是玉米等作物产量损失最具破坏性的原因之一，预计气候变化导致干旱的严重程度和持续时间会增加，这将对农业生产力构成迫在眉睫的威胁。为了了解干旱响应，通常在干旱开始后的给定时间点进行表型和分子研究，代表稳态适应响应。因为生长是一个动态过程，所以我们以高时间分辨率监测干旱反应，并在第四叶出现后 4 天和 6 天检查补水后的细胞和转录组变化。这些数据表明，分裂区活性是补液后器官完全生长恢复的决定因素。此外，PLASTOCHRON1 的异位表达对细胞分裂的长时间维持延长了再水化后恢复生长的能力。转录组分析还表明，生长调节因子 （GRF） 通过影响细胞分裂持续时间来影响叶片生长，再水化后 GRF1 过表达系的延长恢复潜力证实了这一点。最后，我们使用多重基因组编辑方法来评估转录组研究中最有前途的差异表达基因，从而将基因空间从 40 个基因缩小到 7 个基因，以便进行将来的功能表征。

Division zone activity determines the potential of drought-stressed maize leaves to resume growth after rehydration 

Abstract

Drought is one of the most devastating causes of yield losses in crops like maize and the anticipated increases in severity and duration of drought spells due to climate change pose an imminent threat to agricultural productivity. To understand the drought response, phenotypic and molecular studies are typically performed at a given time point after drought onset, representing a steady-state adaptation response. Because growth is a dynamic process, we monitored the drought response with high temporal resolution and examined cellular and transcriptomic changes after rehydration at 4 and 6 days after leaf four appearance. These data showed that the division zone activity is a determinant for full organ growth recovery upon rehydration. Moreover, a prolonged maintenance of cell division by the ectopic expression of PLASTOCHRON1 extends the ability to resume growth after rehydration. The transcriptome analysis also indicated that GROWTH-REGULATING FACTORS (GRFs) affected leaf growth by impacting cell division duration, which was confirmed by a prolonged recovery potential of the GRF1-overexpression line after rehydration. Finally, we used a multiplex genome editing approach to evalsuate the most promising differentially expressed genes from the transcriptome study and as such narrowed down the gene space from forty to seven genes for future functional characterization.

WIWAM line

All traits on the RIL, pGA2ox::PLA1 and GRF1Rlines were measured in a series of experiments in a maize automated Weighing, Imaging and Watering Machine (WIWAM Line). This automated phenotyping platform was designed in collaboration with SMO bvba (Eeklo, Belgium), is located in a controlled-environment growth chamber with the same environmental conditions detailed above and enables automated weighing and

irrigation of plants according to a preset scheme, specific for each plant or group of plants. In this system, up to 155 maize plants can be grown until V7 and can be watered with 1 ml precision to a fixed volume or to varying target weights by watering the plants in a circular motion around the stem at approximately 5 cm distance from the plant. Briefly, a robotic arm takes a pot from the platform and brings it to the weighing and watering station. While watering, the pot is rotated until the target weight is reached. Subsequently, the robotic arm places the pot back on the platform and proceeds to the next pot. The robot pushes the lines of the table aside to create the required space in between for the robotic arm to locate a pot and lift it out of the table. The WIWAM Line can be adjusted for different growth rooms, crops, pot sizes and nutrient solutions, and the weighing and watering station can be equipped with an imaging station where pictures of each plant can be taken daily to monitor plant growth.