欧亚国际

SMO构建WIWAM XY高通量植物表型成像平台

WIWAM植物表型成像系统由比利时SMO公司与GHent大学VIB研究所研制生产，整合了LED植物只能培养、自动化控制系统、叶绿素荧光成像测量分析、植物热成像分析、植物近红外成像分析、植物高光谱分析、植物多光谱分析、植物CT断层扫描分析、自动条码识别管理、RGB真3D成像等多项先进技术，以优化的方式实现大量植物样品以优化的方式实现大量植物样品——从拟南芥、水稻、玉米到各种其它植物的生理生态与形态结构成像分析，用于高通量植物表型成像分析测量、植物胁迫响应成像分析测量、植物生长分析测量、生态毒理学研究、性状识别及植物生理生态分析研究等。

核糖体失活蛋白 OsRIP1 的过表达调节水稻中的茉莉酸酯信号通路

核糖体失活蛋白 （RIP） 是靶向 rRNA 的植物酶。细胞质 RIP 称为 OsRIP1，在调节茉莉酸（一种关键的植物激素）中起着至关重要的作用。了解 OsRIP1 的作用可以为增强抗逆性和优化水稻生长提供视角。通过 mRNA 测序进行转录谱分析来测量响应 MeJA 处理的水稻植物基因表达的变化。与野生型 （WT） 植物相比，当暴露于 MeJA 处理 3 小时时，OsRIP1 过表达水稻植物表现出与茉莉酸反应相关基因的 mRNA 转录物增加较低。MeJA 暴露 24 小时后，与 WT 植物相比，与赤霉素通路相关的 mRNA 转录物在 OsRIP1 过表达植物中的水平较低。我们假设 OsRIP1 拮抗 MeJA 诱导的芽生长抑制的潜在机制涉及细胞分裂素介导的叶片衰老和细胞周期过程的正调节，可能是通过 OsRIP1 与 40S 核糖体蛋白 S5 和 α-微管蛋白的相互作用。此外，光系统 II 10kDa 多肽被鉴定为 与 OsRIP1 有利结合，其参与可能归因于在早期时间点 （3 h） 受到 MeJA 的 OsRIP1 过表达植物光合作用的减少。

关键字

核糖体失活蛋白、水稻、OsRIP1、茉莉酸酯信号传导、光合作用

Overexpression of the ribosome-inactivating protein OsRIP1 modulates the jasmonate signaling pathway in rice

Ribosome-inactivating proteins (RIPs) are plant enzymes that target the rRNA. The cytoplasmic RIP, called OsRIP1, plays a crucial role in regulating jasmonate, a key plant hormone. Understanding the role of OsRIP1 can provide insights into enhancing stress tolerance and optimizing growth of rice. Transcription profiling by mRNA sequencing was employed to measure the changes in gene expression in rice plants in response to MeJA treatment. Compared to wild type (WT) plants, OsRIP1 overexpressing rice plants showed a lower increase in mRNA transcripts for genes related to jasmonate responses when exposed to MeJA treatment for 3 h. After 24 h of MeJA exposure, the mRNA transcripts associated with the gibberellin pathway occurred in lower levels in OsRIP1 overexpressing plants compared to WT plants. We hypothesize that the mechanism underlying OsRIP1 antagonization of MeJA-induced shoot growth inhibition involves cytokinin-mediated leaf senescence and positive regulation of cell cycle processes, probably via OsRIP1 interaction with 40S ribosomal protein S5 and α-tubulin. Moreover, the photosystem II 10kDa polypeptide was identified to favorably bind to OsRIP1, and its involvement may be attributed to the reduction of photosynthesis in OsRIP1-overexpressing plants subjected to MeJA at the early timepoint (3 h).

KEYWORDS

ribosome-inactivating protein, Oryza sativa, OsRIP1, jasmonate signaling, photosynthesis

After treatment with 100 mM MeJA, 14-day-old rice plants of WT, OsRIP1-OE line J and line H were cultured in a plant chamber at 28°C with 16 h light: 8 h dark. Plant health development was monitored longitudinally (0, 3, 6, 9, 24 and 48 h) through multispectral imaging analysis, including efficiency of photosystem II (Fv/Fm) (Baker, 2008), chlorophyll index (Chlldx) (Gitelson et al., 2003), modified anthocyanin reflectance index (mARI) (Gitelson et al., 2009) and biomass approximation based on the number of pixels occupied by the plant. At each timepoint, side-view images

were captured to provide a more precise view of rice plants and leaves (n = 15 plants, 3 plants/image). At each timepoint 15 plants were used for the side-view images and discarded after the image acquisition. All the images were captured by a custom-build

multispectral imaging- and microdispenser platform, equipped with WIWAM system and 6-Mp 16-bit 3CCD top-viewer camera (PhenoVation B.V., Wageningen, The Netherlands). Data processing was performed using the “Data Analysis Software” program (PhenoVation B.V.). Additionally, the phenotypic differences between WT and OsRIP1-OE transgenic plants were analyzed for biomass, shoot length and root length after 48 h of mock treatment or MeJA treatment, respectively

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