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Aquation研究论文-利用原位水下叶绿素荧光仪估计初级生产力
发表时间:2019-02-20 15:59:07点击:1567
Aquation公司总裁与同事发表了利用利用原位水下叶绿素荧光法估计初级生产力的论文,题为 “Estimating primary productivity of marine macroalgae in East Antarctica using in situ fluorometry ”,文章研究用到了Aquation公司的水下叶绿素荧光仪。相关摘要请参见下文。
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Productivity in marine systems has been typically conducted by measuring the flux of oxygen and/or CO2 within enclosed chambers underwater for 24 hour intervals. This can be time consuming and expensive. Simpler modulated chlorophyll fluorescence measurements have long been suggested as a more convenient proxy for productivity, but differences between estimates of carbon fixed per photon derived from gas exchange and chlorophyll fluorescence tend to differ widely in high light conditions. In this paper, the relation between the two is determined over a wide range of light intensities. The derived empirical relation is then applied to in situchlorophyll fluorescence data (effective quantum yield ( )) and irradiance as PAR. Estimates of photosynthesis in the day and respiration in the night are calculated for samples examined only for chlorophyll fluorescence and ambient irradiance. The results show the technique is effective in gathering productivity data in environments were only the deployment of a fluorometer and PAR sensor is required. While the data for the paper was collected at Casey, one of Australia’s continental Antarctic Research Stations, the approach is applicable to most aquatic and terrestrial algae and plants. The Aquation submersible fluorometers are well suited to regularly taking these fluorescence and irradiance measurements.
Abstract
Photosynthetic activity of marine macroalgae in the Windmill Islands, East Antarctica, was measured in situ using submersible modulated fluorometers. An empirical relation incorporating terms for respiration and non-linear electron transport was derived from simultaneous in vivo measurements of effective quantum yield ( ) and oxygen evolution. This relation was used with in situ measurements of and photosynthetic photon flux density acquired over 24-h periods to estimate oxygen evolution rates of algae over the course of the measurement period. Productivity ranged from −8 to 19 µmol O2 g−1 FW h−1 (FW = fresh weight), with daily carbon gain ranging from −1.5 to 3.6 mg C g−1 FW d−1 for midnight ice-covered algae and midday ice-free algae, respectively. These values were similar to published values of productivity of Antarctic species derived from oxygen- and carbon-based techniques. The technique described here provides a simple and rapid means of estimating primary productivity in marine systems.