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Videometer Lab多光谱成像番茄种子研究中的应用
发表时间:2016-12-26 11:45:04点击:2076
种子成熟度是测定种子质量较重要的一个标准,成熟种子拥有强劲的发芽能力,与未成熟种子相比,较能承受胁迫。但基于果实发育阶段,很难测定番茄种子成熟度。以前对与叶绿素含量相关种子外壳颜色研究表明其与种子成熟度存在正相关。
图1. 番茄种子多光谱成像 (a), 背景分离(b)。每个种子标识为“萌发” (红色) 或 “未萌发” (蓝色) -基于在第7天的发芽统计(c) ; nCDA-转换图像(d)。
图1
多光谱成像技术是一种新出现的技术,可应用在种子科学领域,在番茄变化识别上取得了良好的结果。同时我们用化学计量法-标准化的典型判别分析(nCDA)检查了MSI测量番茄种子成熟度的适用性。图 2. nCDA 区分训练集萌发和未萌发的番茄种子(a), 来自青番茄和红番茄的种子(b),横线 (-) 指示了分类的阈值。
图 2
结果表明多光谱成像在种子成熟度测量上的潜力。结果显示种子成熟度测量应该基于种子而非果实成熟或颜色的判断。我们会进一步研究来观测在正常生长条件下的来自青番茄的成熟种子性能。研究链接参见ISTA_Poster_Santosh_For_Print-min.pdf
Use of multispectral images and chemometrics in tomato seed studies
Seed maturity is one of the important criteria for determining the seed quality as the mature seeds possess higher ability to germinate and withstand stresses as compared to immature seeds. However, it is difficult to determine the tomato seed maturity based on the developmental stage of the fruit.1 .Previous studies on seed coat colour pertaining to the chlorophyll content have shown positive correlation with seed maturity.2
Multispectral imaging (MSI) in seed science is an emerging technology and has shown promising results for tomato varietal identification.3 We further aim to examine the applicability of MSI to determine the tomato seed maturity using chemometric method normalised canonical discriminant analyses (nCDA)
The study demonstrates the potential of multispectral imaging for seed maturity determination. The result suggests seed maturity determination should be based on seeds instead of judging by fruit maturity or colour. However, further investigation would be required to see performance of the mature seeds from the green tomatoes under normal growing conditions.