欧亚国际

较近，来自比利时根特大学VIB所等机构的科学家，WIWAM植物表型成像系统发表了题为Robust increase of leaf size by Arabidopsis thaliana GRF3-like transcription factors under diferent growth conditions的文章，对器官生长的基因进行了研究。VIB所的Dirk Inze教授团队一直致力于“Organ Size Control”的研究，前不久刚刚就PLA1促生长基因的研究发表了先进论文。

WIWAM植物表型成像系统开发班底来自VIB、CropDesign以及SMO公司，其表型成像技术代表了较先进水准。北京欧亚国际科技有限公司是比利时WIWAM植物表型成像系统的中国区总总代理，全面负责其系列产品在中国市场的推广、销售和售后服务。

Robust increase of leaf size by Arabidopsis thaliana GRF3-like transcription factors under diferent growth conditions

Matías Beltramino1, María Florencia Ercoli 1, Juan Manuel Debernardi1, CamilaGoldy1,Arantxa M. L. Rojas1, Florencia Nota2,3, María ElenaAlvarez 2,3, LiesbethVercruyssen4,5, Dirk Inzé 4,5, Javier F. Palatnik1,6 & Ramiro E. Rodriguez 1,6

An increase in crop yield is essential to reassure food security to meet the accelerating global demand. Several genetic modifcations can increase organ size, which in turn might boost crop yield. Still, only in a few cases their performance has been evalsuated under stress conditions. MicroRNA miR396 repress the expression of GROWTH-REGULATING FACTOR (GRF) genes that codes for transcription factors that promote organ growth. Here, we show that both Arabidopsis thaliana At-GRF2 and At-GRF3 genes resistant to miR396 activity (rGRF2 and rGRF3) increased organ size, but only rGRF3 can produce this effect without causing morphological defects. Furthermore, introduction of At-rGRF3 in Brassica oleracea can increase organ size, and when At-rGRF3 homologs from soybean and rice are introduced in Arabidopsis, leaf size is also increased. This suggests that regulation of GRF3 activity by miR396 is important for organ growth in a broad range of species. Plants harboring rGRF3 have larger leaves also under drought stress, a condition that stimulates miR396 accumulation. These plants also showed

an increase in the resistance to virulent bacteria, suggesting that the size increment promoted by rGRF3 occurs without an obvious cost on plant defenses. Our fndings indicate that rGRF3 can increase plant organ size under both normal and stress conditions and is a valuable tool for biotechnological applications.